Characterization of potato (Solanum tuberosum)lactate dehydrogenase isoenzymes by affinity chromatography and hybridization.
نویسنده
چکیده
The five isoenzymes of potato (Solanum tuberasum) lactate dehydrogenase have been resolved by affinity chromatography. Mixtures of isoenzymes LDH-1 and LDH-5 dissociate and reassociate during freezing and thawing to produce five isoenzymes. These results indicate that potato lactate dehydrogenase isoenzymes are primary isoenzymes of the vertebrate type, which are composed of two subunit types.
منابع مشابه
Distribution of lactate dehydrogenase isoenzymes in potato (Solanum tuberosum) and other plants.
A, digestive gland; B, buccal cavity; C, mantle muscle; D, eye; E, brain. The lower margin of the photograph is the position reached by bromophenol blue. The L-LDH was specifically isolated by affinity chromatography before application to the tops of gels (see text). Electrophoresis was carried out in tubes of 7.5% (w/v) polyacrylamide at pH 8.3 according t o the method of Davis 1.51. LDH was v...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 197 3 شماره
صفحات -
تاریخ انتشار 1981